The effect of electromagnetic field of acoustic range on kinetics of enzyme-substrate reactions and rheological properties of blood
Project Status: 3 Approved without Funding
Duration in months: 24 months
Objective
The Project aim: In in-vivo and in-vitro experiments on animals:
1. To determine the effect caused by Electromagnetic Fields of Acoustic Range (EMFAR) on: a) rheological properties of blood in the microcirculatory system; b) kinetics of certain enzyme-substrate reactions;
2. To study and analyze possible physiological, biochemical and biophysical mechanisms taking part in developing the uncovered effects;
3. To work out the certain recommendations and algorithms for using EMFAR in medicine and biology;
4. To develop mathematical models on the basis of procured experimental data, which, if confirmed as adequate, will make it possible to conduct deeper and more multi-faceted analysis of events observed while using electromagnetic fields, in both clinical and experimental conditions. Current status: The widespread use of EMFAR in order to intensify chemo-technological processes, as well as experiments conducted and appropriate methodologies developed in our country provide a plausible basis to expect that the fields of specified range, when handled adequately, can be effectively used for treatment of various diseases. However, while employing any treatment method, it’s crucial to not only determine its effectiveness, but also identify mechanisms of its action and correction on this basis of the dosage and duration of use, based on the probability and severity of side effects.
Low Frequency (LF) magnetic fields currently are widely used for the treatment of various diseases. This solidifies the high topicality of the study of mechanisms of influence and possible impacts of such emissions on living organisms. A certain difficulty, but at the same time a great scientific interest of conducting such researches are also connected to the fact that to this day there are no theoretical models or hypothetical assumptions regarding the mechanisms of action of LF magnetic fields on living systems. The project’ influence on progress in this area: The most critical novelty of the proposed project is that the authors suggest and are intending to not only expose the effects of the impact of given fields on living organisms, but also uncover the physiological, biochemical and biophysical mechanisms, mediating these effects specifically in case to changes in the blood’s rheological properties and/or the kinetics of enzyme-substrate reactions.
We hope that the received results will allow consciously and purposefully use this non-invasive and not pharmacological method for treatment of a broad range of diseases associated at least with disturbances in the rheological properties of blood and intensity of the enzyme-substrate reactions. The participants’ expertise: The high competence of the project participants is confirmed by numerous publications and successfully conducted grant projects with their direct involvement and leadership. Multidisciplinary scientific team (consisting of experienced physiologists, biochemists, physicists and mathematicians) is able to use not only modern physiological and biochemical approaches for research, but also actively employ the powerful apparatus of electromagnetic field theories, mathematical modeling, mathematical statistics and theoretical physics that will allow to use possibilities of both analysis as well as synthesis of the systems under study. Technical approach and methodology: Experimental study will be carried out on white rats in norm and disturbed rheological properties of blood, as well as normal and disturbed production of Nitric Oxide. Different parameters of EMFAR will be used. All received results will be evaluated statistically and mathematical models of different stages of the observed processes will be created. Based on experimental data and mathematical simulation special recommendations and algorithms for using of EMFAR in clinic will be developed.
For experimental research of kinetic systems, the Michaelis-Menten method will be employed. As a substrate we use a hydrogen peroxide, as the enzyme – catalase, as the noncompetitive inhibitor – copper sulfate, and as the competitive inhibitor – ascorbic acid.
In order to rate the influence of biochemical system parameters and the EMFAR on the speed of reaction and subsequent planning of experiments, the dispersive analysis method of Fisher will be used. As for defining the dependence forms between biochemical and physical values, methods of correlative and regressive analysis will be applied.
Participating Institutions
LEADING
I. Beritashvili Center of Experimental Biomedicine (IBCEB)
COLLABORATOR
Valley Cancer Institute
COLLABORATOR
Duke University/Medical Center
COLLABORATOR
Argonne National Laboratory (ANL) / Advanced Photon Source
COLLABORATOR
Harvard Medical School / Beth Israel Deacones Medical Center
