Genetic research of filterable forms of Mycobacterium and effort to find new therapeutic preparations
Project Status: 3 Approved without Funding
Duration in months: 36 months
Objective
The Project aim. Develop a new therapeutic drug against Mycobacterium tuberculosis on the basis of the interfering RNA Current status. At present, the experimental data on the biological properties of the filterable forms and the L-forms of Mycobacterium bovine species and developed their way of accumulating bacterial mass in a nutrient medium. The project’ influence on progress in this area. The use of interfering RNA may be one of the effective tools in the fight with complex infectious disease, such as multi-resistant tuberculosis. The participants’ expertise. The participants’ expertise. Scientists involved in the project have extensive experience in the design, implementation and production of diagnostic, preventive drugs to protect animals against tuberculosis, as express methods for laboratory diagnosis of tuberculosis. Expected results and their application. Use of interfering RNA can appear one of effective remedies in struggle against difficult infectious diseases, such as the multiresistant form of a tuberculosis.
As a result of project performance will be:
- studied the cultural-morphological properties of filtered forms of mycrobacteria;
- defined stable and medicinal-steady genes of filtered forms of mycrobacteria;
- selected optimal genes of mycrobacteria for their blocking;
- constructed the short two-chain RNA complementary to a gen of mycrobacteria;
- constructed a protein-polymeric liposomic preparation on a basis of interfering RNA;
- conducted the preparation tests in vitro and in vivo on laboratory animals. Meeting the ISTC goals and objectives. As the project participants are experts in the field of mass-destruction weapons and the project has exclusively peace orientation, it quite corresponds to ISTC objectives. Also, compliance to these purposes is reached at the cost of planned wide involvement of scientists from participating organizations in the world scientific community by presentation of the project data at international conferences and seminars. Scope of activities. The following main works will be executed within the project:
- allocation of filterable forms of mycobacteria and explore their cultural and morphological properties;
- study of the genetic characteristics of filterable forms of mycobacteria;
- construction of RNA interference;
- selection and formation of liposomic preparation;
- testing of the drug to the culture of mycobacteria;
- testing of the drug on laboratory animals. Role of Foreign Collaborators/Partners. Consultations, organization of business trips for preliminary interconnection with other partners, trainings, scientific exchange and coordination of joint actions, joint workshops (including working panel), meetings and consultations, technical report analysis, joint use of rare materials, samples and means, joint or parallel studies, discussion of obtained information, verification of results using self-contained methods and technology, joint publications and official registration of right fir intellectual property. Technical approach and methodology. Under the Project will be conducted the following works: 1) to isolate filterable forms of Mycobacterium and study its cultural-morphological properties. Filterable forms will be isolated from classical forms of Mycobacterium by cultivation in nutrient medium and by infecting laboratory animals. 2) To study the genetic characteristic of filterable forms of Mycobacterium. Position of stable and drug-resistant genes will be defined. 3) Construction of interfering RNA. Gene site responsible for drug-resistance will be defined and constructed two-chain RNA complementary to this site. 4) Selection and construction of a liposomic preparation. Selection of ratio for phosphatidylcholine, cholesterol, dicetylphosphate and polyethylene glycol for obtaining microcapsules. Application of tubercular antibodies for liposomes. 5) Preparation test on Mycobacterium cultures. Testing the preparation for bacterial and bacteriostatic actions. 6) Testing the preparation on laboratory animals. Efficiency of the preparation will be testedonr laboratory animals preliminary infected with TB.
