Assessment of the current situation of sheep and goat pox in Kyrgyz Republic: Development, improvement facilities and methods of virus detection
Project Status: 3 Approved without Funding
Duration in months: 24 months
Objective
Kyrgyz Research Institute of Veterinary named after A. Duysheev (KRIV) program identifies specific diagnostic antigens and produces specific antibodies to a wide range of critically important interest in animal diseases and toxins. The experience, previously gained by KRIV and its collaborators during the past forty years, in developing diagnostic tests and medical countermeasures against a variety of economically important livestock diseases will be used in this program.
Timely prophylaxis and measures of specific prophylaxis play basic role in struggle against smallpox of animals. In the world to make prophylaxis of sheep and goat pox, vaccines from attenuated strains of pox virus are used. At a choice of the vaccine designed for mass immunization, special attention is paid to safety and duration of immune defense of animals after using vaccine. To obtain safe virus vaccine various systems of cultivation is used and optimum conditions are selected.
However many questions bound to cultivation of a virus of a smallpox, still are insufficiently studied. For reception of virus various systems were used, the majority from which have appeared unsuitable, that has been bound to its low reproduction or loss of immunogenic features of the virus. Reception of the virus with high titer on cell cultures LK, GK, LТ and GТ is possible by optimization of culture conditions at which its initial biological features remain. Besides it is supposed using of transplantable of cell cultures ВНК-21 (a kidney of the Syrian hamster), GG (gonads of a goat), LK (Lamb kidney), GK (Goat kidney) from which the optimal variant for cultivation will be chosen for the purpose of maximum harvesting. The decision of this problem is an actual task.
One of the important points in struggle against sheep and goat pox is fast and exact diagnosis when disease appearing, and as consequence the operative measures referred to liquidation of the nidus of infection.
At the project to define disease clinical signs and serological methods of diagnosis of sheep and goat pox as reactions: AGID, reaction neutralization (RN), enzyme-linked immunosorbent assay (ELISA), with use of specific serums and the antigens developed for each reaction are used.
Modern methods ELISA and PCR have a number of basic features in comparison with traditional: quality and standardization of used antigens and the antibodies possessing higher activity and specificity, especially at use of the cleared materials (antigens). The basic advantages of a method also are high sensitivity, simplicity of conducting, productivity.
Development of diagnostic of sheep and goat pox and definition of the immune status concerning a smallpox are proved by that in modern conditions when well-being of the country on a smallpox becomes perceptible, infection contamination drift is not excluded, and therefore timely diagnosis and decoding of an etiology of sick rate of each single instance get the special importance and an urgency. But polymerase chain reaction (PCR) is one of the basic additional methods of diagnosis which has certain disadvantages. For example, variability nucleus sequences of a genome field isolates the virus and insignificant contamination can lead to formation of false negative or false positive results. Moreover, the molecular-genetic methods of diagnosis based on direct identification of the virus or its genetic material, are applicable at early stages of disease whereas immunologic methods and immunochemistry methods are used as for antigen revealing, and virus antibodies, do not limit times of selection of a pathological material or assays of blood sera.
The questions on developing of improvement of measures and methods of laboratory diagnosis of sheep and goat pox are actual questions with regard to the above-stated.
In the end of the two-year-old program developing and introduction of diagnostic kits for sheep and goat pox the most suitable test systems will be selected, conducting of reaction and estimating reagents will be optimized. The developed test systems will be accompanied by all necessary reference documents and certificates and to be assumed for use in diagnostic laboratories and practicing veterinary experts.
Participating Institutions
LEADING
Kyrgyz Research Institute of Veterinary named after A.Duysheev (KRIV)
COLLABORATOR
Colorado State University / College of Veterinary Medicine and Biomedical Sciences
