Study of Biochemical Changes in Infected Cell System for Evaluation of Biological Activity of Viruses

Tech Area / Field

• BIO-CHM/Biochemistry/Biotechnology

Status
3 Approved without Funding

Registration date
19.07.1999

Leading Institute
The Republican Government Enterprise on the basic of economic control rights “Research Institute for Biological Safety Problems” , Kazakstan, Gvardeiski

Project summary

The objective of the proposed project is study of biochemical changes in infected cellular system for determination of their possible correlations to infection multiplicity.

For implementation of this task it is planned to study biochemical changes in culture system of “SPEV” cells infected with rinderpest virus. It is supposed to study changes of lipid, nucleic, protein and amino acid metabolism. While studying lipid metabolism changes quantitative parameters of the major lipid precursor ¹⁴C-sodium acetate inclusion in infected cells depending on infection dose will be determined (including use of purified rinderpest virus and taking into account lipid composition of bovine blood serum). Studies of nucleic metabolism changes in rinderpest virus infected cell culture system will proceed from “SPEV”cells ability to include RNA precursor ³H-uridine. Variations in radio-labelled uridine inclusion in infected and control cells will be determined in presence of actinomycin C which blocks cellular RNA synthesis and with use of homogeneous and heterogeneous scintillation systems. Protein metabolism changes will be studied by quantitation of ¹⁴C-labelled protein compounds in maintenance media of infected and control cells. Moreover, influence of rinderpest virus on amino acid content in maintenance media of virus producing and control systems will be studied. All the findings will be used in determination of correlation between the changes under study and infection multiplicity; on this base method of rapid evaluation of biological activity will be developed according to model “rinderpest virus-SPEV cells”.

Successful implementation of this project will result in scientific precedent solving the problem of rapid and less labour-consuming evaluation of biological activity of viruses.