Early Nonphototoxic Luminescence Diagnostic of Cancer
Early Luminescence Diagnostic of Endoscope Accessible Forms of Cancer for Risk Groups of Population Based upon Creation and Using of Nonphototoxic Photosensitizers of Malignant Tissue and High Sensitive Stroboscopic Oncofluorometer
Tech Area / Field
- MED-DID/Diagnostics & Devices/Medicine
8 Project completed
Senior Project Manager
Safronova O N
Russian Academy of Sciences / Institute of Radioengineering and Electronics / Fryazino Branch, Russia, Moscow reg., Fryazino
- Scientific and Technological Center of Unique Instrumentation, Russia, Moscow\nState Research Institute of Biological Instrument-Making, Russia, Moscow\nCancer Research Center, Russia, Moscow
- Illinois State University, USA, AL, Normal\nUniversity of Padova / Department of Biology, Italy, Padova\nUniversity of Cologne / Clinic of Nuclear Medicine, Germany, Köln\nWashington University School of Medicine, USA, MO, St Louis
Project summaryThe goal of the project is development of the basis of the early nonphototoxic diagnostics of endoscope accessible forms of cancer. Following tasks should be solved in course of the project.
1. Development of new group of photosensitizers (PS), namely nonphototoxic one, which should act as diagnostic substance. Unlike well known therapeutic photosensitizers, such as Photofrin-II, Foscan, Photohem, Photosence (Phthalocyanin), Chlorine-E6, etc., new drugs must not generate singlet oxygen in human body, while providing high degree of tumorotropy and luminescence contrast, and prolonged luminescence lifetime. All those features, in combination with other necessary spectral characteristics, make possible use of increased concentrations of nonphototoxic photosensitizers and higher levels of excitation radiation.
2. Development of specialized diagnostic laser-fiber-based apparatus providing stroboscopic detection and processing of signals. In particular, high sensitive mobile oncofluorometer based on acousto-optic tunable filters (AOTF) should be created, which is applicable to clinics and diagnostic rooms, including traveling medical vans.
First samples of diagnostic photosensitizers (metal-porphyrin complexes) comprising ions of Ytterbium or Platinum have been synthesized, tested, and patented by some of the authors (Patents of Russian Federation No. 1340087 and 1707539). There basic properties, which are nonphototoxicity, luminescence contrast exceeding 100, high degree of tumorotropy, validate backgrounds of the project.
In the course of the project, the detailed investigations of nonphototoxic photosensitizers to be synthesized will be held in vivo and in vitro using biological simulators and animals. Diagnostic facility is based on tailor-made (specialized) stroboscopic installations and acousto-optic oncofluorometer. Guidelines and instructions for medical personnel on practical using nonphototoxic PS and diagnostic equipment will be developed.
The project has to resolve following 5 problems.
1. Development and synthesis of porphyrin metallocomplexes promising for early luminescence diagnostic (ELD) of cancer.
2. Preliminary investigation of spectral luminescence characteristics of nonphototoxic photosensitizers.
3. Development of specialized diagnostic electronic apparatus.
4. Research of behavior and properties of nonphototoxic photosensitizers inside biological objects.
5. Development of diagnostic luminescence-detection procedures. Equipment testing in experiments including animals.
It requires approximately 4780 person day of efforts from weapon scientists and engineers (category 1) and 2108 person day of efforts from the other personnel (category 2).
The project must result in development of diagnostic certified photosensitizers production technology, creation of mobile stroboscopic oncofluorometer (MS OF) and approvement it efficiency to hospitals, preparation of guidelines and instructions for doctors, and technical documentation for MS OF pilot-scale production.