Development of Live Recombinant Vaccine Against Legionellosis
Tech Area / Field
- BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
8 Project completed
Senior Project Manager
Evstyukhin K N
State Research Center for Applied Microbiology, Russia, Moscow reg., Obolensk
- FOA National Defence Research Establishment, Sweden, Stockholm
Project summaryDevelopment of experimantal samples of live vaccine against legionellosis on the base of Francisella tularensis recombinant vaccine strains synthesining protective antigenes of is Legionella pneumophila the major goal of the project proposed.
The following problems to realise the project properly should be solved :
- to identify and study L.pneumophila antigenes which seems to be promising for the construction of F. tularensis recombinant strains;
- to select appropriate test systems for L. pneumophila antigenes;
- to perform the cloning of L. pneumophila genes encoding the protective antigenes;
- to develop vector systems providing the transition, expression and stable inheriting; of legionella genes in the cells of F. tularensis;
- to construct recombinant strains of F. tularensis synthesizing protective antigenes of L. pneumophila;
- to study immune and biological properties of the recombinant strains obtaind by using the model of legionellosis infection;
- to select the recombinant strains of F. tularensis promising as the experimental samples of the vaccine
It is expected that protective antigenes of L.pneumophila will be identified a characterized, their encoding genes will be cloned, and recombinant strains of F. tularensis for the legionellosis antigenes production will be constructed as a result of the investigatic The strains promissing as experimetal samples of vaccines will be selected on the basis of immunobiological properties; the experimental samples of antilegionellosis vaccines will created.
The realization of the project proposed should yield a novel approach to the construc-tion polyvalent recombinant live vaccines on the base of the vaccine against tularemia, that general seems to be a new trend in the construction of live recombinant vaccin Experimental data obtained will be published and extend the knowlege of scientists in field of immunology and genetics of L. pneumophila and F. tularensis. The experimental vaccine samples will be used in medicine practice for tprevention of Legionaires1 disease.
Our recent studies on the optimization of the method of F. Tularensis transformatio construction of the vector plasmids on the basis of natural plasmide isolated from. novicida like strain F5969, construction of F. tularensis strains synthesizing protective antigenes of bacilla as well as the set of the cloned L. pneumophila genes encoding legionellosis antigenes should assist us in solving the problems in hand.
The project proposals address the objectives of ISTC in providing weapons scientist a: engineers opportunities to redirect their talents to peaceful activities. In particular, the experimental antilegionellosis vaccine can be used for medicine and health care needs.