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Immunosensor for Bioagents detection

#2467.2


Biosensor for Biological Pathogens Rapid Detection on the Basis of Immunoluminescence Microassay

Tech Area / Field

  • MED-DID/Diagnostics & Devices/Medicine
  • BIO-SFS/Biosafety and BioSecurity/Biotechnology
  • MED-DIS/Disease Surveillance/Medicine
  • ENV-MIN/Monitoring and Instrumentation/Environment

Status
3 Approved without Funding

Registration date
04.08.2005

Leading Institute
State Research Institute of Biological Instrument-Making, Russia, Moscow

Collaborators

  • SENOVA GmbH, Germany, Jena\nLondon School of Hygiene&Tropical Medicine, UK, London\nIOM GmbH, Germany, Berlin

Project summary

Objective of the project – to develop an active sample of biosensor device for rapid automatic detection of biological agents in environmental specimens (air, water) as well as in other specially prepared materials, e.g. food products, clinical samples etc.

Feasibility of Direction of Research

This project is based upon the use of Phosphorescence Analysis (abbreviated PHOSPHAN) – the technology, which has been recently developed at FSUE “GOSNII BP” and defended by a number of Russian patents. In the PHOSPHAN technology, a new type of label is used – the phosphorescent Pt coproporphyrins (Pt CP) capable of long-living luminescence (phosphorescence) in their conjugates with proteins (antibodies, streptavidin). This technology allows ultra low analyte concentrations to be detected directly from the solid surface of polymeric materials in reactions of immunological binding. The use of Pt-CP provides high detection sensitivity (up to 5 103 molecules of Pt-CP per 1000 μ2 area) in time-resolved mode that allows single microbe cells to be detected under scanning the solid surface covered with agent-specific immunoreagents.

The principle of biosensor operation includes a forced absorbtion of biological objects (BO) to a solid water-permeable membrane followed by their detection in reactions of immunological binding by measuring the luminescence intensity with a scanning immunophosphorescence analyzer.

In the framework of this project we plan to create a biosensor model, and then a full-size device, for the detection of multiple agents of dangerous infectious diseases (such as anthrax, plaque, tularemia and other) in water and air samples.

Direction of Research:

· Modeling the technical portion of biosensor device.


· Develop biological components for biosensor testing.
· Develop techniques and a timing diagram (cyclogram) of analysis.
· Select and evaluate technical parameters of the full-size transducer components.
· Create an active model of full-size transducer, and validate its analytical and performance characteristics.
· Update the transducer in accordance with testing results.

Expected Results and Their Application

Expected characteristics of biosensor device are as follows:

· Threshold limit of bioagents detection in water samples (50-200ul), no more than 100 microbes (cells/sample)


· Maximum number of the detected types of bioagents - 9
· Period of full cycle analysis, min - 15
· Period of continuous operation (without replacement of expendable reagents), days - 10
· Cost of a serial sample of device – 45,000 $US.

In the framework of this 2-year project we are going to create a prototype of automatic biosensor device, and after its testing to prepare a plan of putting into serial production both the device and expendable reagents.

Role of Collaborators

Joint discussion of project results; meetings at conferences and direct visits, providing comments on the project reports and technical assistance (if required), preparation of cooperative reports for conferences and publications are the main points of the collaboration.


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