Ferric Hydrophosphate Against Infectious Diseases
Ferric Hydrophosphate – Preparation of New Generation Against Infectious Diseases
Tech Area / Field
- CHE-SYN/Basic and Synthetic Chemistry/Chemistry
- MED-DRG/Drug Discovery/Medicine
3 Approved without Funding
Center for Coordination and Implementation of Scientific, Technological and Socio-Economic Projects, Georgia, Tbilisi
- Georgian Academy of Sciences / P. Melikishvili Institute of Physical and Organic Chemistry, Georgia, Tbilisi\nTbilisi State Medical University / Research Institute of Experimental and Clinical Medicine, Georgia, Tbilisi
- Northern Kentucky University, USA, KY, Highland Heights\nGlobal Technology Transfer, USA, KY, Convington
Project summaryThe main objective of the Project is research into the bactericidal and viral properties of ferric hydro-phosphate and a determination of its application in vivo in the treatment of infectious diseases.
Humans live in areas which are filled with various microorganisms (bacteria, microbes, and viruses), with the majority of those causing all sorts of infectious diseases, with no remedy found as yet. Since the viruses invade and live inside a cell and they are involved in the cell metabolism of an organism the diseases they cause create a bigger problem than the bacterial infections. Antibiotics of various groups are widely applied in modern medical practice but the clinical problems, such as tolerance (i.e. special ferments generated by the bacteria), ruin the antibiotics and decrease their effectiveness. These are becoming common occurrences. Another big problem is the allergic reactions that occur frequently in an antibiotic therapy. The foregoing makes the search for anti-microbe medicines chemically alien to antibiotics a hot issue. The anti-microbe medicines manufactured from organic ferric compounds are characterized with high toxicity and a microbes have already developed resistance against them.
We have developed a method of preparation for an aqueous ferric hydro-phosphate solution. This marks the first attempt at an application of an inorganic ferric hydro-phosphate in medicine (regardless of iron’s long determined function of erythrocytes in hemoglobin formation, as well as that of ion phosphate PO43- viewed as “physiological”). The phosphate ion, PO43-, is regarded as being one of the most important anions in living organisms. This is due to its significant role in maintaining an acid-alkaline balance of blood through a formation of a phosphate buffer system.
We can state that aqueous solutions of ferric hydro-phosphate is not toxic, based on our earlier findings and importantly it possesses anti-microbial and anti-viral properties.
We have studied the bactericidal properties of ferric hydro-phosphate on various microorganisms that cause plague, dysinteria, typhus, pseudo-tuberculosis, an intestinal bacillus and iercines, a cholera vibrion, golden staphylococcus, klebsiella and salmonella, as well as the stems of flu, coccsac B, hepatitis A and B, and the West Nile fever.
The research results testify to a complete loss of infecting (virulent) properties of flu during 6 min. at 0.02% concentration; of West Nile fever – during 3 min at 0.02% concentration; of the coccsac B virus – during 6 min. at 0.1% concentration; of the hepatitis A virus – during 6 min. at 1% concentration; of the golden staphylococcus microbes – during 6 min. at 0.5% concentration.
Thorough research into the ferric hydro-phosphate with an application of toxicological, patho-morphological, histological, patho-physiological, neurological and immunological methods testify to loss of high toxicity in peroral application. Importantly, it does not have a cumulative effect.
We have also detected a possible in-vivo application of the ferric hydro-phosphate of various concentrations. The in-vivo experiments on rabbits have shown non-formation of necrotic areas on the inner organs both in one-time as well as with repeated injections in-vivo of the 0.25% and the 0.5% ferric hydro-phosphate solution. We have also done histological research; the histological structure of parenchymathose organs have not been disrupted. Furthermore with sufficient blood supply we have observed no disruptions of blood circulation.
Thus, the preliminary research has testified to the bactericidal and antiviral effects of ferric hydro-phosphate and its low toxicity. Significantly, the fact that ferric hydro-phosphate was applied in-vivo is important. A number of thorough and applicable tests (thorough research into) envisaged by the Project will open the way for a creation of a new generation anti-microbial medicine for a treatment of infectious diseases.
The Project requires research into ferric hydro-phosphate in the following directions:
– Preparation of aqueous solutions of bi- and tri-valent iron in various concentrations;
– Study of their physical-and-chemical properties of the above solutions;
– Study of the mechanisms through which ferric hydro-phosphate affects microbes and viruses;
– Determination of its effective concentration;
– Determination of its toxic and carcinogenic properties;
– Determination of the effect the elements (Ca, Mg, Zn, Al, etc.) have on its anti-microbial and anti-viral properties;
– Model tests on animals using in-vivo injections of the aqueous solutions of ferric hydro-phosphate;
– Creation of a non-toxic new-generation medicine for a treatment of highly infectious and chronic diseases;
– Research into its penetration through the cell membranes and physiological barriers (haematoencephalic and placental);
– Research into the distribution of the medicine in the organs and tissues of an organism;
– Determination of its pharmacological-and-kinetic parameters;
– Research of its bio-transformation;
– Research into the bio-accessibility of ferric hydro-phosphate.
In order to achieve the objectives of the Project, complex research using modern methods for physical and chemical analysis such as spectrophotometric, X-graphic, spectral electronic microscopes as well as a chromatography (high pressure solution chromatography by HPLC, thin-layer TLC, gas-solution GLC, and chromato-mass-spectrography GC/MS) is envisaged.
Involvement of competent researchers (participants) is required to ensure a success of the Project. The co-authors of the Project have long-term experience in studies of synthesis and research into the hydro-phosphates of certain elements and medical applications thereof. They have been successfully involved into the INTAS Program (No.97-30443) between 1999-2002. The thesis was titled: “The Study of The Calcium Hydro-phosphate and Its Application in Medicine”. Six patents related to the properties of ferric hydro-phosphate were granted to them in Georgia. Undoubtedly this testifies to the competence of the researches. The research team is engaged regularly in conferences and symposiums, notably the 1998 NATO symposium in Georgia of 1998.