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Biosensor for Bacteria Detection

#3101


New Type of Biosensor for Bacteria Detection Using Phages

Tech Area / Field

  • BIO-MIB/Microbiology/Biotechnology

Status
3 Approved without Funding

Registration date
17.08.2004

Leading Institute
State Research Center for Applied Microbiology, Russia, Moscow reg., Obolensk

Collaborators

  • United States Department of Agriculture / Agricultural Research Service / Food Technology and Safety Laboratory, USA, MD, Beltsville\nUniversité de Claude Bernard-Lyon 1 / Laboratoire de Sciences Analytiques UMR 5180, France, Villeurbanne\nUniversitat de Barcelona / Department of Microbiology, Spain, Barcelona\nLaval University, Canada, QC, Quebec City\nUniversity of Helsinki / Haartman Institute, Finland, Helsinki\nConsejo Superior de Investigaciones Cientificas / Centro Nacional de Microelectronica, Spain, Bellaterra\nUniversità degli Studi di Firenze / Dipartimento di Chimica, Italy, Florence\nTufts University, USA, MA, Medford\nUniversity of California / Department of Mechanical and Aeronautical Engineering, USA, CA, Davis\nUniversity of Tennessee / Center for Environmental Biotechnology, USA, TN, Knoxville\nUniversity of Guelph / Canadian Research Institute for Food Safety, Canada, ON, Guelph\nTokyo Institute of Technology / Department of Biotechnology, Japan, Tokyo

Project summary

In this project we propose to use phages as a new recognition system for biosensor construction. Phages are viruses that infect bacteria with multiplying inside them by making use of some or all of the host biosynthetic machinery. Phages are used in the diagnostic laboratory for the identification of pathogenic bacteria (phage typing). But this typing is not used in the routine and need at least a day for cultivation. The tail fibers attach to specific receptors on the bacterial cell and the host specificity of the phage is usually determined by the type of tail fibers that a phage has. The nature of the bacterial receptor varies for different bacteria.

The main idea of this project is the specific and partial disruption of the cell envelope of pathogen bacterial cells by phages and the registration of those injuries by adding metabolic substrates or dyes which are not penetrate to native bacterial cells. Adding of metabolite substrate or dyes to specific disrupted cells could induce oxygen consumption or reduction of dyes which are simple to detect by biosensor. This method will be very important in a wide range of disciplines, including medical analysis, food and environment. It will provide us with a real opportunity to develop and apply to practice specific technologies associated with application of phages to rapid and sensitive analysis of bacterial world.

Our team has had a core competition in microbiology and sensorology. In SRC AM works in the field of bacterial detection are intensively conducted. The results of theses investigations were published in international per review journals (Biosensors and Bioelectronics) and were presented at different conferences (including NATO ARW and NATO ASI).


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