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Molecular characterization of swine influenza viruses

#K-2231


Molecular characterization of swine influenza viruses circulating in different regions in Kazakhstan and comparison with contemporary human influenza viruses

Tech Area / Field

  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology

Status
6 Project underway

Registration date
06.12.2015

Senior Project Manager
Asainova S T

Leading Institute
Institute of Microbiology and Virology, Kazakstan, Almaty

Collaborators

  • St. Jude Children's Research Hospital, USA, TN, Memphis

Project summary

The issue of interspecies transmission of swine and human influenza А(Н1N1) viruses is an important factor in studying evolution, ecology, and epidemiology of the pathogen. There are theoretical justification of probability of interspecies transmission of influenza A virus between birds and sea animals, birds and swines, seals and humans, swines and humans. Emergence of reassortants with new properties and mechanisms causing adaptation of influenza viruses of birds and animals to human body remains unknown [Hinshaw V.S. et al.1983; D.K. Lvov, 1998].

Subject to the methodological approaches to implementation, this Project is a follow-up of the earlier virology research of influenza A viruses and is aimed to obtain new results in the area of molecular biology and natural evolution variability of influenza A viruses of different etiology.

The proposed project provides for identification of new pathogen serovariants, determination of their antigenic relationships, nucleotide sequences of НА and NA genes, and intrinsic proteins of new Kazakhstani strains of Human and Swine influenza А/Н1N1 viruses for a comparative phylogenic analysis. The processes of their molecular genetic variability have not been discovered so far, and researches in this area are highly relevant in the Republic of Kazakhstan, since they will allow obtaining important data relating to the regularities of the evolution of influenza pathogens, in general. Newly isolated original Kazakhstani influenza A virus strains will be used as a basis for obtaining purified and high-avid antigens recommended as test-systems for diagnostics of influenza infection. The research findings can be used for interpretation of influenza outbreaks and control of arising emergency epidemiological situations in the Republic of Kazakhstan.

The potential users of the obtained results are the Animal Health Committee of the Ministry of Agriculture of the Republic of Kazakhstan, Committee for State Sanitary and Epidemiological Supervision of the Ministry of Health of the Republic of Kazakhstan.
The Project results will be of interest for the general scientific community and professionals in the area of practical healthcare and veterinary medicine.
The main point of the Project concerns to determine the prevalence and characteristics of influenza viruses in swine in Kazakhstan and to identify zoonotic and reverse zoonotic transmission events.
To obtain the Project goal, the following tasks should be accomplished:
Objective 1: Identification and characterization of influenza viruses circulating in swine in different regions in Kazakhstan.
Objective 2: Characterization of influenza viruses collected from humans in regions where swine surveillance is conducted.
Objective 3: Phylogenetic characterization to identify interspecies transmission events between humans and swine.


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