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F. Tularensis Genes and Antigens

#2945


Comparative Analysis of Francisella Tularensis Genes and Antigens of Different Biovars

Tech Area / Field

  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
  • BIO-MIB/Microbiology/Biotechnology

Status
3 Approved without Funding

Registration date
03.12.2003

Leading Institute
State Research Center for Applied Microbiology, Russia, Moscow reg., Obolensk

Supporting institutes

  • Institute of Bioorganic Chemistry, Russia, Moscow

Collaborators

  • Umeä University / Department of Clinical Bacteriology, Sweden, Umeä\nOklahoma State University / College of Veterinary Medicine, USA, OK, Stillwater

Project summary

Study of F. tularensis antigens of different biovars is the major objective of the project. The biovars will be obtained from N. F. Gamaleya Institute for Microbiology and Epidemiology collection.

Isolation of lipopolysaccharide and protein antigens is planned to be performer within the project framework. The antigens will be obtained by using immunochemical and physicochemical techniques. Monoclonal antibodies will be developed in order to analyse the isolated antigen preparations. Whole genome comparative analysis of different F.tularensis serovars is planned to be carried out by using techniques of subtracting hybridization. Libraries of differences between serovars will be developed which will allow the serovars identification as well as discovering of genetic determinants of antigenic specificity.

F. tularensis biovars will be described in their antigen structure and genetics simultaneously for the first time. Furthermore, development of the monoclonal antibodies will provide good opportunities for detailed study of structure and function of the Tularensis cell proteins as well as for differentiation of its various geographic races. The whole genome comparative analysis will allow estimate genetic differences of biovars more objectively and make conclusions at the level of proteome and antigenic determinants. The present works will provide an essential gift into fundamental and applied biology, immunology and medicine.


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