Role a Bacterial Antistressors in Virulence and Intracellular Parasitism of Legionella Pheumophila, Francisella Tularensis and Salmonella Typhimurium.
Tech Area / Field
- BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
8 Project completed
Senior Project Manager
Evstyukhin K N
State Research Center for Applied Microbiology, Russia, Moscow reg., Obolensk
- University of Colorado / Health Science Center, USA, CO, Denver\nIowa State University of Science and Technology / Ames Laboratory, USA, IA, Ames
Project summaryThe purpose of the project is the identification and comparative analysis of the stress-response genes and their products which allow bacterial pathogens such as Legionella pneumophila, Francisella tularensis and Salmonella typhimurium to function as parasites in eukaryotic cells. Identifying the genes which encode this ability may make it possible to use them to create effective live vaccines by means of subtle genetic modification of the pathogenic properties of intracellular parasites.
Achieving this goal will require the following:
1. The creation of adequate conditions and methods for in vitro and in vivo induction and detection of the stress-response products, particularly proteins and cyclopyrophosphates of Legionella pneumophila, Francisella tularensis and Salmonella typhimurium.
2. Generation of isogenic strains Legionella pneumophila, Francisella tularensis and Salmonella typhimurium harbouring mutations in gens for intracellular parasitism.
3. Comparative analysis of stress-response synthesis in mutants with different degrees of ability to function as intracellular parasites, in order to identify the products most important for the bacterial' survival in eukariotic cells and to characterize physiological state of mutants within eukariotic cell.
4. Cloning and sequencing the structural and regulatory genes which encode both the universal and the specific stages of intracellular parasitic infection by these pathogens.
5. Identification of the basic structures and regulatory genes which encode stress-response products in various modes of intracellular parasitism.
The project will be staffed by highly-qualified scientists and engineers previously involved in defence projects, who will apply the methods of molecular biology to the investigation of the pathogenesis of infectious diseases while working on their own equipment in their own laboratories.