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Europium Fluorescence Sensitizer

#0623


Synthesis of Complexion of Europium Fluorescence Sensitizer

Tech Area / Field

  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
  • BIO-CHM/Biochemistry/Biotechnology
  • BIO-OTH/Other/Biotechnology

Status
8 Project completed

Registration date
16.04.1996

Completion date
13.04.2000

Senior Project Manager
Nietzold D

Leading Institute
State Research Institute of Biological Instrument-Making, Russia, Moscow

Collaborators

  • Molecular Probes, Inc., USA, OR, Eugene\nUniversity of Maryland at Baltimore, USA, MD, Baltimore\nUniversity of Washington, USA, WA, Seattle\nUniversity of California / College of Agricultural and Environmental Sciences, USA, CA, Davis\nIGEN International, Inc., USA, MD, Gaithersburg\nArgonne National Laboratory (ANL), USA, IL, Argonne\nUniversity of California / Calvin Laboratory, USA, CA, Berkeley

Project summary

The Project Goal

The main goal of project is the increasing of sensitivity in undissociative time resolve immunofluorescent assay.

Technical Approach

Changing the scheme of immunological determination from dissociative on undissociative by means changing the reagents for analysis. The synthesis and investigation of new class of fluorescent markers, in that the properties of binding to proteins and europium complex formation are joined with effective sensitizing of europium fluorescence.

The new markers will contain the fragment of diethylentriaminpentaaceti■acid for strong binding of complexions with europium ions and fragment of f1uorocontaining aromatic beta-diketones for effective energy transform on europium ions. The molecular-mechanical modeling and calculation for optimal structures determination, synthesis of these structures, investigations of their spectrophysical characteristics and biochemical studies for using in fluorescent time resolving immunoassay will be conduct.

Expected Results

It is supposed to decrease the threshold of marker registration up to 10-14-10-13 mol/1 in proposed method, that correspond to sensitivity of determination - 10-13-10-12 g of protein with molecular mass 150000 in probes.

Approbation of developed reagents on models of toxins and quarantine infections, that it is interested for ISTC and it is coordinated with ISTC.

Potential Role of Foreign Collaborators

The foreign partners can take interest in researches of new markers spectral characteristics and also in biochemical analysis on model and practical systems.


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