Lymphytropic Strain of Amoeba
Lymphotropic Strain of Acanthamoeba. Interactions of Amoeba and Human Cells in vitro and Consequences of Amoeba Penetration into a Thymus
Tech Area / Field
- MED-DID/Diagnostics & Devices/Medicine
3 Approved without Funding
Institute of Immunology, Russia, Moscow
- Institute of Immunological Engineering, Russia, Moscow reg., Lyubuchany
- Harvard School of Public Health, USA, MA, Boston
Project summaryThe goal of the project is to analyze the molecular basis of the interactions between amoebocytes and human lymphoid cells, with special reference to mechanisms of thymocyte apoptosis induced by amoeba strains and their humoral products.
During the last decades, 29 new infectious diseases have been registered, often connected with danger to human life. The manifestation of pathogenic properties in microorganisms and protozoa previously considered as saprophytes is now a regular occurrence. Infectious agents that target lymphoid organs deserve special attention, as the immunodeficiency they induce makes it easy for other pathogens to penetrate into the organism.
Originality of the Project
The Project authors first isolated a CDHT amoeba strain of Acantamoeba genus with a number of extraordinary properties from a thymus of a child under cardiac defect surgery. Those strain cells weakly proliferated in monoculture. Their insertion into thymocyte culture and that of other human cells drastically enhanced their proliferation. Also, thymocites formed aggregates with amoeba cells, underwent apoptosis and were eliminated from the culture, resulting in a cessation of amoebocyte proliferation. Thymocyte apoptosis could be induced by dialyzable humoral factor, isolated by CDHT cells. Amoebocytes induce apoptosis of activated and leukosis T-thymocytes.
Amoeba cells serologically related to CDHT line amoebocytes were periodically found (using antibodies against CDHT cells) in fragments of the thymus of a child subjected to heart surgery, which allowed the supposition of a possible connection of cardiac defect formation with amoeba invasion in the embryonal period. On the other hand, natural antibodies, interacting with CDHT cells, were found practically in the sera of every healthy person. Their titre rise was registered in liquidators of Chernobyl disaster consequences.
· Biochemical characterization of amoebocyte products, which are homologous to thymic hormones.
· Study of the consequences and nature of CDHT (Cells Derived from Human Thymus) cell tropism to lymphoid organs of mice and their ability to disturb immune processes in vivo.
· Estimation of embryotoxicity of CDHT cells and correlation of developmental defects with acantamoeba invasion.
· Development and trial of the test-systems for determination of CDHT-related amoeba cells and antibodies against CDHT surface antigens in sera of aging humans and patients with immunological disorders
Meeting ISTC goals and objectives
Project execution would contribute to the solution of an important social problem, i.e. it would give scientists and engineers from the Institute of Immunological Engineering, Joint-Stock Company “Biopreparat”, who previously fulfilled state orders of the Ministry of Defence, the opportunity to reorientate their scientific interests and to apply their accumulated experience to fundamental and applied studies, connected with the solution of international research and technical problems in biology and medicine for peaceful purposes.