Bacterial and Cell Culture Collection
Reorganization of Bacterial and Cell Culture Collection to Study Infectious Diseases in Humans and Animals
Tech Area / Field
8 Project completed
Senior Project Manager
Weaver L M
State Research Center for Applied Microbiology, Russia, Moscow reg., Obolensk
- Center for Emergency Preparedness and Response Population and Public Health Branch, Canada, ON, Ottawa\nBioterrorism Pesponse - National Microbiology Laboratory Public Health Agency of Canada, Canada, MB, Winnipeg\nCenters for Disease Control and Prevention (CDC), USA, GA, Atlanta
Project summaryRealization of R&D works and organizational and technical activities directed on preservation and development of SRCAM Collection of microorganisms, improving of storage and maintenance conditions of the Culture Collection and support of research programs with standard collection cultures are the major objectives of the project proposed.
Despite of great achievements in the field of microbiology, epidemiology, prevention and treatment of infectious diseases, epidemiological situation in Russia and abroad is unfavorable. During the last years, the incidence of epidemically significant diseases (tuberculosis, AIDS, intestinal infections, etc.) has become very high. Besides, the existing natural foci of infections represent a threat of epidemic outbreaks of especially dangerous infections. The situation becomes aggravated during social catastrophes. Despite the realization of preventive measures, the rise of the incidence of infectious diseases having seasonal prevalence is annually observed. Isolation of microorganism strains possessing drug resistance testifies to constant variability of microbes. The strains resistant to drugs have a special value for practical medicine; therefore constant control of their distribution is necessary. For research of the epidemic process and systematization of the infection sources, it is important to conduct collecting, study and long maintenance of microbial cultures. Special attention should be given to the following strains:
- referent-strains for perfecting standard techniques and methods of works and usage of these strains as taxonomy criteria;
- newly isolated cultures for improving methods of prevention, treatment and diagnosis of infectious diseases;
- strains-producers of useful substances.
During a 25-year activity of the Center, a representative Collection of bacterial, phage strains and somatic cell lines has been established. These biological materials are maintained by currently used methods of preservation and storage (cryopreservation, lyophylization, contact-sorption dehydration). The Collection funds of SRCAM are mainly presented by lyophylized cultures. At present, the works on enlargement of the Collection using cryopreservation methods are in progress.
Within the framework of international programs the developments in the field of monitoring of bacterial agents causing diseases in humans and animals are realized. A number of the projects are executed under the support of the International Science and Technology Center. Much attention is given to the studies on pathogenicity and antibiotic resistance of microorganisms causing infectious diseases.
However, detail studies of the isolated cultures, their systematization and long-term storage are beyond these projects. At the same time, a great number of microorganisms isolated from the objects of the environment and presenting scientific and practical interest are not the objective of the international projects. Thus, during the implementation of a number of international projects, a lot of microbial cultures, such as mycobacteria, enteropathogenic E.coli, campilobacteria, almost 1000 bacillus isolates possessing antagonistic activity against pathogenic microorganisms, etc. have been isolated in different European and Asian regions of Russia. Of importance is not only to study the biological properties of these cultures, but also to place them for a long-term storage. These and other cultures present certain value for study of the biological persity of microorganisms, preservations of genetic resource and searching for strains having applied importance. In SRCAM, the works on establishment and enlargement of bacteriophage and somatic cell line collection are in progress. The expanding international cooperation requires operative exchange of information and microbial cultures between the institutions engaged in the studies. However, principles of preparation to the exchange as well as the procedure of the exchange are distinguished in different countries, that demand the development of unified approaches. Within the project proposed, legislative principles specifying the process of biological material exchange will be studied, and the scheme of the biological material exchange will be developed.
In order to meet the project objectives, the following tasks will be fulfilled:
1. Conduct inventory of SRCAM Collection of microbiology cultures and somatic cell lines.
2. Develop an electronic version to account documentation of the Culture Collection.
3. Give the information about the Collection in the SRCAM Internet site.
4. Study the preservation of biological properties of the bacteria during long-term storage.
5. Update the Collection cultures of bacteria, phages and cell lines.
6. Study biological characteristics of the isolates obtained within the international projects and place the isolates for long-term storage.
7. Perform genotyping of the collected strains using certain species (Bacillus, Pseudomonas, Enterobacteriaceae) as an example.
8. Develop the scheme of Collection culture and information exchange taking into account national and international requirements.
Realization of the project will allow to provide preservation and further development of SRCAM Culture Collection, to provide standard Collection cultures for execution of federal programs and international projects.
The project proposed will allow to use the experience of highly skilled scientists and engineers earlier engaged in the development of biological protection means as well as to use laboratory facilities and equipment for decision of scientific and applied tasks of the public health care.