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Helicobacter Pylori Resistance

#G-904


New Natural Antimicrobial Factors Affecting Helicobacter Pylori and Genetic Mechanism of Antibiotic Resistance in Some Regions of Caucasus

Tech Area / Field

  • MED-DRG/Drug Discovery/Medicine
  • MED-DIS/Disease Surveillance/Medicine
  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology

Status
3 Approved without Funding

Registration date
10.07.2002

Leading Institute
Tbilisi State Medical University / Research Institute of Experimental and Clinical Medicine, Georgia, Tbilisi

Collaborators

  • Vanderbilt University Medical Center, USA, TN, Nashville

Project summary

The main goals of the presented project are:

– to substitute (partially or completely) antibiotics by new natural substances found out by the authors; these substances which should be verified on large amount of experiments are of organic and non-organic origin and they are perfectly harmless for human organism;


– to monitor antimicrobial resistance of local H. pylori strains and to recommend antibiotics of choice for clinics;
– to investigate genetic mechanism of antimicrobial resistance of H. pylori strains obtained in some regions of Caucasus.

At nowadays due to wide spread of more than 50% of world population are infected with this bacterium. It is a well-known fact that this microorganism is a great danger for people in 20-30% cases. It is also known, that the index of infection, caused by H.pylori, depends on geographic areas, ethics and race, factors of habits, sex, age and social environment. It is especially common in economically under-privileged countries.

This infection is mainly spread in the kind of gastroduodenal diseases: chronic gastritis type B, peptic ulcer of stomach and duodenum and MALT-lymphoma, gastric cancer. According to certain literature, the mentioned bacterium causes diseases of other visceral organs of humans.

At widely spread gastroduodenal disease associated with H.pylori the treatment regimes usually include 2-3 antibiotics. This causes disturbance of microbial biocenosis as a result of antimicrobial resistance and other side effects.

According to WHO recommendations, it is urgent to have systematical and international monitoring of antimicrobial resistance of local strains in each geographical region.

In 1997, at the conference on H.pylori in Canada it was decided to have strict monitoring of these infections caused by local antimicrobial resistant H.pylori strains in different regions of the world. Thus, the study of chromosomal and nonchromosomal mechanisms of antimicrobial resistance of local strains H.pylori presents certain interest for theoreticians and practitioners.

Taking into consideration the above-mentioned facts, a great amount of work is under way to find alternative ways of treatment and relational schemes of antibiotic therapy.

In the Department of Molecular Microbiology of the Institute of Experimental and Clinical Medicine at Tbilisi State Medical University the problems of antibiotic resistance and its genetic have been under investigation for over 30 years. More than 100 papers were published both in Georgian and foreign Journals. In particular, the study of H. pylori has been going on since 1994. There where 5 papers published and a seminar of leading specialists was held (Tbilisi, Georgia 2001); natural substances to reduce of antibiotic resistance and destroy H.pylori strains in vitro were found. The authors of the project have good experience in identification and definition of H. pylori and its antibiotic resistance, as well as detection of R-plasmid in antibiotic resistant strains.

Realization of the project will be done in the above-mentioned Department.

The novelty and expected results are:

– detection of possibility to use new natural antimicrobial factors affecting H.pylori on the bases of large amount of observations;


– peculiarities of biological properties of local H.pylori strains;
– detection of local antimicrobial resistance of H.pylori strains and comparison to that of investigations in other countries of the world;
– genetic mechanism of antimicrobial resistance of local H. pylori strains;
– recommendation of different generations choice antibiotics for clinics.

The project gives opportunity to restructure the facilities related to military service in the past to peaceful activity promoting the health of community.

For project realization is necessary to carry out the following activities gradually:

Stage I - to investigate the specific possessions of H.pylori strains a study its multiresistance at Caucasus region.


Stage II - to put in order the new natural antimicrobial A and B factors, acting on H.pylori, found out by ourselves for application in vivo.
Stage III - chromosomal and nonhromosomal antibiotic resistance of H. pylori strains.

Consistant with the scope of activities of the project as described above, specify the scope of cooperation with a foreign institutions:

– information exchange in the course of project implementation;


– consultations on the methods used in the study and help with analysis and presentation or results;
– provide comments to the technical reports (quarterly, annual, final, etc.), submitted by project participants to the ISTC;
– provide necessary consultations and help on this project and help with preparation of this proposal;
– joint discussion of obtained results at working seminars;
– joint attendance of international meetings.

Technical approach and methodology are:

– for transportation of studied material to laboratory would be used the special transport media;


– incubation and obtaining of strains H.pylori would be carried out in microaerophillic environment;
– for identification of H.pylori would be used the following methods: microbiological, cytological, histological, respiratory urease with isotope and rapid urease test;
– resistance of strains to antimicrobial agents would be studied by recently applied methods.

Study of R-plasmid spreading among H.pylori antibiotic resistant strains and physical and chemical properties of R-plasmid would be performed by methods of molecular microbiology and genetic engineering.


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