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Artificial Nose System for Bacteria Detection

#3256


Remote Method for Bacteria Detection with Using an Artificial Nose System Based on High-Density Optical Biosensor Arrays

Tech Area / Field

  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology

Status
3 Approved without Funding

Registration date
14.04.2005

Leading Institute
State Research Center for Applied Microbiology, Russia, Moscow reg., Obolensk

Collaborators

  • Matforsk, Norway, As\nUniversität Tübingen, Germany, Tübingen\nThe University of Warwick / The School of Engineering, UK, Coventry\nUniversity of California / Department of Mechanical and Aeronautical Engineering, USA, CA, Davis\nUniversitat Regensburg / Institute of Analytical Chemistry, Chemo- and Biosensors, Germany, Regensburg\nUniversité de Limoges, France, Limoges\nUniversity of Limerick / College of Informatics & Electronics, Ireland, Limerick\nTufts University, USA, MA, Medford\nUniversity of Toronto, Canada, ON, Toronto

Project summary

This project is devoted to the use of up-to-date remote method for bacteria detection with using an artificial nose system based on high-density optical biosensor arrays for the real-time microbes detection. This method will allow to detect bacteria without contact with biological material and will be very important in a wide range of disciplines, including medical analysis, food and environment.

Our team has had a core competition in the microbiology (SRC AM). We are intensively working in the field bacterial biosensors. The results of our work published in international per review journals (Biosensors and Bioelectronics) and presented at different conferences (including NATO ARW and NATO ASI).

The results of the investigations will enable us to open the new field of the bacteriology – aroma microbiology, and how smell of biological sample is correlated with the habitation environment and specific features of bacterial composition. They will provide us with a real opportunity to develop and apply to practice specific technologies associated with application of fiber-optic biosensor to rapid and sensitive analysis of bacterial world.

The scope of the project will include the following activities:

  1. Prepare of fiber-optic sensor system based on the multi-bead arrays for odor analysis
  2. Analysis of volatile components for classification of different bacterial strains.
  3. The study of growth phase during bacterial cultivation by using artificial nose system.
  4. Quantitative detection of bacteria during cultivation.
  5. Compare of volatile components from the dead bacteria and from the alive cells
  6. Develop a two-format (etched and cover slip) technology to produce bead based fiber optic arrays.
  7. Develop recommendations for microbiological analysis of the samples with using of the artificial nose system.


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