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Immunosensor for Bioagents Detection

#2467


Biosensor for Biological Pathogens Rapid Detection on the Basis of Immunoluminescence Microassay

Tech Area / Field

  • MED-DID/Diagnostics & Devices/Medicine
  • BIO-SFS/Biosafety and BioSecurity/Biotechnology
  • ENV-MIN/Monitoring and Instrumentation/Environment

Status
8 Project completed

Registration date
27.03.2002

Completion date
04.10.2005

Senior Project Manager
Melnikov V G

Leading Institute
State Research Institute of Biological Instrument-Making, Russia, Moscow

Project summary

Aim of the project – to create a model of biosensor system for biological agents rapid detection which will be a part of a transducers operating in independent mode during 3-6 months.

The project is based on the use of long luminescing (phosphorescing) Pt coproporphyrins as luminescent labels of antibodies in reactions of immune binding. These labels provide much more high sensitivity of detection in comparison with commonly used ones.

In the frames of the project (2 years) it is supposed to create analytical portion of sensor for simultaneous detection of several (no less 4) biological agents (BA).

Biosensor should be capable of specific detection up to 100 cells being immobilized by antibodies on the area, 5-10 mm2 in size.

Principle of assay is biospecific BA adsorption from water to solid polymer surface (tape), covered by immobilized antibodies, followed by bound BA interaction with detector antibodies labeled with Pt coproporphyrin. After removing of unbound antibodies, the signal of prolonged luminescence will be measured in time resolved mode in microsecond decay time range of the label.

At the end of the 1st year of the project lifetime, the following results should be obtained:

Working model of analytical portion of biosensor (detection unit) should be created followed by demonstration its availability for purposes and tasks of E-smart-2000 program. Biosensor should be capable of 4 biological agents simultaneous detection over 15 min time period.

A number of simultaneously detected BA could be expanded significantly because of no principal limitations in the technology of solid phase carrier fabrication (polymer tape with separate zones of immobilized antibodies).

Sensitivity of detector system should provide the detection of 100 to 1,000 cells of pathogenic bacteria per ml of water. Biosensor should be capable of BA detection in amounts no less than 50-100 cells after their adsorption to solid phase in zones of biospecific binding.

Biosensor should be capable of antigenic determinants detection on the surface of microbe cells and unable to differentiate alive and dead cells.

Sensitivity of detector system for biological toxins detection will be no more than 1...5 ng/ml. Taking into account trial results with different protein models, the expected threshold detection level could be as small as 0.05-1 ng.

Analytical portion of biosensor could be fully automated and is capable of BA detection with periodical connection to E-SMART network in every 15 min. Management and controlling could be also conducted by E-SMART network.

The project is very important for meeting ISTC goals and objectives.

The project supports applied investigations and the development of newest technology of biological microassay which are directed to realization the programs of ecological monitoring of the environment for dangerous infectious diseases.


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