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Adjuvants of veterinary vaccines

#KR-2042


Development of production technology and delivery systems for novel adjuvant of veterinary vaccines

Tech Area / Field

  • AGR-VTH/Vaccines and Theraupetics/Agriculture
  • BIO-SFS/Biosafety and BioSecurity/Biotechnology
  • CHE-POL/Polymer Chemistry/Chemistry

Status
3 Approved without Funding

Registration date
20.09.2012

Leading Institute
Kyrgyz Research Institute of Veterinary named after A.Duysheev, Kyrgyzstan, Bishkek

Supporting institutes

  • National Academy of Sciences of Kyrgyzstan / Institute of Chemistry and Chemical Technology, Kyrgyzstan, Bishkek

Collaborators

  • University of Oslo / Department of Pharmacy, Norway, Oslo\nPacific Northwest National Laboratory, USA, WA, Richland

Project summary

The goal of the project is to develop technologies for formulation and usage of novel and highly effective vaccines with potential to enhance immnunity of animals against foot-and-mouth (FMD) and sheep pox diseases. Our previous studies suggest that for the production of vaccines with enhanced immunogenic effect the most promising seems to be the production of adjuvant polysaccharide-based vaccines with multifunctional properties. Biological natural polymers as adjuvant substances and delivery carriers have many advantages, including good biocompatibility, nontoxicity, and adjustable controlled release properties.
Polysaccharides from plants have been the subject of studies for a very long time, mainly focused on their physical properties, their chemical and physical modification, and their application. Over the last decade years there has been increasing interest in the biological activity of the natural polysaccharide polymers. These studies became possible as a result of the scientific development of isolation, purification and characterization methods concomitant with the development of fairly simple in vitro tests for effects especially on the immune system (Smestad and Barsett, 2005). We will focus on two polysaccharides to be used as adjuvants for vaccines: pectin and inulin.
Pectins have a variety of pharmacological effects, such as immunostimulating, anti-metastatic, anti-ulcer, anti-nephrosis activities, and cholesterol-reducing effects amongst others. Pectins have been used in drug delivery, as adjuvant in vaccines, and also how the fine structure of each polymer is important for the activity shown of the specific polymer and concludes with observations on how pectins can be used in many ways for the benefit for human beings (Berit, Barette, 2005).
Inulin has many health benefits, it increases calcium and possible magnesium absorption, while promoting the growth of intestinal bacteria (Abrams et al., 2005). A combination of prebiotic short and long chain inulin-type fructans enhances calcium absorption and bone mineralization of young adolescents (Corday et al., 2003).
These objectives will be accomplished through a series of integrated studies designed to:
- isolate polysaccharides, namely pectin and inulin from the sugar beet and plants of Linula и Consinia genera accordingly, determination of the structure of the isolated pectin and inulin. Determination in detail the relevant bioactive part of the isolated pectin;
- isolate of viruses of FMD and sheep-pox from a pathological material;
- formulate vaccine compositions based on bioactive agents (in concentration inducing physiological response) and the polysaccharides (pectin, inulin). To develop formulations in various forms (liquids, solids);
- estimate of immunological response of animals vaccinated with sheep pox and FMD adjuvant vaccines. To perform a comparative analysis among new developed and current vaccines.
To types of vaccines will be developed using different techniques for vaccines' formulation. To formulate FMD vaccine the inactivated cultural cells will be composed with polysaccharide-based adjuvant for immunity enhance. For alive attenuated virus for sheep pox vaccine the using of adjuvant will keep stability for a long time.
Thus, the developed compositions can be intended for increase of immune responses of animals, stability and/or increase of storage time of sensitive preparations. The in situ formed gel can slow down and/or modulate the release of preparation, on one hand, and to improve biological availability of physiologically active agents, on other hand.
Publications relevant to the project are listed below:
Ashubaeva, S.D., Kozlova I.I. 1991: Metalcomplex derivatives of pectins and their use. Bishkek, Ilim, 94 pp.
Aimukhamedova, G., Aliyev D., Shelukhina N. 1984: Properties and application of pectin sorbents. Frunze, Ilim, 116 pp.

Aimukhamedova G. et al. 1999: Potassium polygalacturonate with chemiosensitizing tumor effect. Kyrgyz prepatent, 318.
Turdumambetov K., Usubalieva G., Jorobekova Sh.J. Study of С. Sporadocephala Jus carbohydrates and composition setup of their glucofructans. Proceedings of NAS KR. Bishkek, 1999, 2, 34-38.
Turdumambetov K., Usubalieva G., Jorobekova Sh.J. Composition setup of С.Lieocephala (PFL) Jus glucofructans. Proceedings of NAS KR. Bishkek, 2000, 2, 32-35.
Turdumambetov K. Study of production kinetics of glucofructans from plant raw material. Proceedings of NAS KR. Bishkek, 2005, 4, 54-58.
Turdumambetov K. Oligo- and polysaccharides of С.Tianschanica plants and study of chemical composition. Proceedings of NAS KR. Bishkek, 2008, 2, 34-37.
Turdumambetov K., Usubalieva G., Jorobekova Sh.J. Production method for inulin. Patent of KR, No 331, 1999.
Zakharova N., Kydralieva K., Khudaibergenova E., Jorobekova S. et al. Synthesis of nanosized pectin-based formulations. Macromolecular Symposia. 2012, 317-318, 175–179
Kydralieva K., Koroleva R., Usupbaeva C., Zakharova N., Jorobekova Sh. Development of metal-containing nanoscale pectin-based materials for adjuvant chemotherapeutics. Proc. of Int. Congress "Nanotechnology in Biology and Medicine" (BioNanoMed2012), 01-02 March 2012, Krems, Austria.


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