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Scanning Microscopy for Living Cells Monitoring

#1849


In-situ dynamical monitoring of living bacteria cells using scanning probe microscopy

Tech Area / Field

  • BIO-MIB/Microbiology/Biotechnology
  • MED-DID/Diagnostics & Devices/Medicine

Status
3 Approved without Funding

Registration date
12.04.2000

Leading Institute
Moscow State University / Department of Chemistry, Russia, Moscow

Supporting institutes

  • Scientific-Practical Materials Research Centre NAS of Belarus, Belarus, Minsk\nNational Academy of Sciences of the Republic of Belarus / Institute of Physical Organic Chemistry, Belarus, Minsk

Project summary

Modern methods of scanning probe microscopy open new possibilities to study morphology and properties of living bacteria cells. Probe microscopy allows to visualize nanometer-scale details of structure on the surface of a living cell, provides information about mechanical (adhesive, frictional, viscosi-elastic etc.) properties of cellular wall and what is even more important, to observe processes occurring to inpidual cells such as growth, pision, lysis, movement, aggregation into colonies and other time-taking changes of cells' state in natural environment - air or liquid. The main features of probe microscopy listed above open new perspectives of solving fundamental and practical problems of microbiology and medicine and unlike conventional high resolution electron microscopy allows to work with living cell cultures.

The main task of the proposed project is the development of express method of studies, characterization and diagnostics of bacteria cells by means of scanning probe microscopy. Previously, the authors have systematically studied enterobacteria for different taxonomical groups (Eshirichia coli, Klebsiella, Helicobacter pylori, Bifidobacterium etc.), and also hybrid bacteria, constructed by means of gene engineering. The following main tasks are to be accomplished under the project:

- to create comparative atlas of 3D images of bacteria for express diagnostics in practical microbiology and medicine,

- to work out new methods of probe monitoring of dynamical processes - growth, pision and lysis of bacteria cells,

- to carry out systematical studies of adhesive properties of cell cultures with respect to materials used in medicine,

- to work out methods of chemical modification of materials surface by thin organic films for decrease of bacteria adhesion and, on the contrary, creation of materials with strong adhesion due to biospecific binding,

- to work out methods of monitoring the state of bacteria cells when subjected to drugs, disinfecting and washing liquids,

- to register lysis of inpidual cells when attacked by bacteriofags,

- to charaterise living vector vaccines created by means of gene transduction,

- to elaborate new instrumentation and specialized devices of probe microscopy for biological monitoring and express analysis of bacterial strains.

On the basis of the accomplished project instructions on the use of probe microscopy in practical medicine for microbiological express analysis will be worked out.


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