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Novel Biosensors

#0890


Novel Biosensors Based on Bacterial Cell and their Fragments

Tech Area / Field

  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
  • AGR-DIG/Diagnostics/Agriculture

Status
8 Project completed

Registration date
19.03.1997

Completion date
21.10.2003

Senior Project Manager
Nietzold D

Leading Institute
State Research Center for Applied Microbiology, Russia, Moscow reg., Obolensk

Collaborators

  • Potsdam University, Germany, Potsdam\nTufts University, USA, MA, Medford

Project summary

Actuality

The requirement for accurate analytical information is particularly marked in human health care, veterinary medicine, and environmental monitoring. Biosensors promise to provide analytically powerful and inexpensive alternatives to standard chemical and enzymatic methods used today.

The aims of this Project are:


- the development of a new generation of biochips based on bacterial cytoplasmic membranes (CPM)
- the creation of novel biosensor devices with high sensitivity and stability using dormant bacterial cells and/or CPM with modified surface charge
- the application of novel biosensors in medical, ecological and food technology analyses

For realization of this aim we plan to solve next problems:


1. To carry out the induction of specific enzyme activity in bacteria for analysis of new biological substrates and to select optimal conditions for that.
2. To obtain bacterial CPM with specifically enzymatic activity for biochip creation.
3. To study the possibility for increasing of sensitivity and stability of biosensor devices by modification of CPM surface charge using negatively and positively charged modificators.
4. To obtain dormant bacterial cells by using starvation methods.
5. To evaluate the possibility of application of dormant cells for biochip creation.
6. To elaborate new methods for medical diagnostics, environmental monitoring and control of food quality using new biosensors.

Planned results


- new generation of biochips based on bacterial CPM will be developed
- the stable and sensitive biosensor will be fabricated using genetic, chemical and physiological approaches during biochip preparartion
- the development of the new biosensor devices to evaluate the milk quality
- the creation of the biosensor for bacterial enumeration in different liquid
- new biosensors for anionic detergents will be obtained
- the fabrication of the new system for lactate detection in the blood

Analysis time (< 5 min) and inexpensive fabricated devices will be very attractive for commercial aims.

Preliminary work


- The work with biosensor devices has been already performed by participants of the project
- Participants of the Project possess extensive experience in cultivating and studying bacteria as well as preparing CPM from microorganisms
- The possibility for modification of surface charge of bacterial membranes has been shown
- Previous data for possibility to use bacteria for biochip creation have been obtained.

This work is in line with the goals of the International Scientific and Technical Center, as it enables skilled specialists earlier engaged in the defense R&D to direct their activity towards solution of fundamental and applied problems for medical diagnostics, environmental monitoring and control of food quality using new biosensors.

Foreign collaborators:

Up to now, foreign scientists from University of Venice (Italy) and Potsdam University (Germany) expressed their interest in collaboration in the frame of this project.

Project participants are ready for collaboration in the frame of the ISTC project. Expected types for collaboration:


- exchange of information;
- review of technical reports;
- sharing of materials and samples;
- joint seminars, workshops, meetings, consultations;
- verification of the obtained results by collaborators using their approaches and/or equipment;
- joint and/or parallel investigations;
- collaboration on I PR protections;
- collaboration on commercialization of the results.


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