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Diagnostics of Oxidative Stress

#3317


Express Diagnostics of Oxidant/Antioxidant, SH - S-S and Micro Element Balance of Human Organism with the Use of Electrochemical Sensors and Measuring Instruments

Tech Area / Field

  • MED-DID/Diagnostics & Devices/Medicine
  • MED-OTH/Other/Medicine
  • CHE-ANL/Analytical Chemistry/Chemistry

Status
3 Approved without Funding

Registration date
07.07.2005

Leading Institute
Ural State Economic University, Russia, Sverdlovsk reg., Ekaterinburg

Supporting institutes

  • All-Russian Center of Emergency and Radiation Medicine named after A.M. Nikiforov, Russia, St Petersburg

Collaborators

  • Universität Hamburg / University Hospital Hamburg-Eppendorf / Institut für Allgemeine und Klinische Pharmakologie und Toxikologie, Germany, Hamburg\nArgonne National Laboratory (ANL) / Center for Environmental Restoration Systems, USA, IL, Argonne\nTETRAD Technologies Group, Inc., USA, VA, Richmond\nNanoCarbon Research Institute Ltd., Japan, Chiba\nUniversidad Complutense de Madrid, Spain, Madrid

Project summary

Insolation, ionizing radiation and xenobiotics may cause excess formation of free radicals and, therefore, may lead to the oxidative stress. The oxidative stress largely contributes to the development of some diseases of the cardiovascular and nervous systems, lungs, eyes, blood, and senescence of the human organism.

The protection system includes enzymes, vitamins, thiol-containing amino acids, flavonoids, carotinoids, etc. Most of these substances possess the antiradical (antioxidant) activity. The rapid determination of this activity and the knowledge of the microelemental balance of the organism provide diagnostic information about the state of the organism (the oxidant stress) and are very useful for selection of specific therapeutic treatment and assessment of its efficiency, especially during surgical operations.

The existing methods for determination of the level of the oxidative stress in the human organism, assessment of the general antioxidant activity (AOA) of various biological materials and evaluation of the ratio between oxidized and reduced forms of thiols (TDR) and the balance of microelements (MEB) are laborious and time consuming. Also, they require highly qualified personnel and expensive equipment for their performance.

The project work will help solve a topical problem, which consists in development of inexpensive commercial equipment and new readily available methods for rapid assessment of the oxidant/antioxidant balance (AOA and TDR) in biological systems and evaluation of the concentration of microelements (MEB) in the human organism. Then it will be possible to determine indications to appropriate therapeutic treatment and monitor the efficiency of this treatment and prescribed medicines. This is especially important in surgery.

The project work will provide new very valuable information for medicine and will result in creation of novel diagnostic methods and devices lending themselves to a wide use.

High professional skills of scientists (which are confirmed by a large number of their frequently cited publications), physicians and engineers, who will be involved in the project work, wide opportunities and the experience in estimation of the oxidative stress by modern methods (ARCERM of EMERCOM of Russia and Medical Pediatric Academy S-Petersburg), a large experience in development of electrochemical measurement means (USUE), results of preliminary analysis of AOA, TDR and MEB in real biological materials (blood and its fractions) using electrochemical methods (Central Clinical Hospital SB of RAS and USUE), and a long-term experience and competence of foreign collaborators instill confidence in the successful fulfillment of the project tasks.

Large sensitivity, high selectivity and rapid acquisition of results, which are ensured by electrochemical methods, provide the possibility to adapt of the relatively simple instruments developed in terms of ISTC Projects Nos. 342 and 2132, and use it as prototypes for fulfillment of the aforementioned tasks. The following commercial devices will be designed: an antioxidant tester, an analyzer of the thiol/disulfide ratio and the microelemental balance in the organism, sensors and analysis algorithms. AOA, TDR and MEB will be analyzed in clinical conditions using biological materials taken from patients exposed to radiation, those having an acute cardiac pathology, and in the perioperative period.

The project offers opportunities to scientists and specialists, who earlier executed orders from the RF Ministry for Defense, to turn to peaceful activities, ensures the integration of Russian scientists into the international scientific community, and attracts the intellectual potential and research abilities of the project contractors to the corresponding activities of the international community. The project supports basic and applied research and development of high technologies in medicine.

The project facilitates the development of the market economy and satisfies civil requirements since the international market badly needs equipment for quick prenosological diagnostics, preventive therapy, evaluation of the condition of patients, and the choice of appropriate therapeutic treatment during emergencies. The successful commercialization of the equipment and the sensors, which were developed under ISTC Projects Nos. 342 and 2132, gives good outlooks for the similar successful commercialization of results obtained in terms of the proposed project.

It is planned that foreign collaborators will take part in experiments, discussion of results, and commercialization of the equipment.


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